ASWJP

RECIPE FOR 1 LITER

Before starting make the following stock solutions:

Stock		ingredient		g / 100 ml

#1		KCl			5.5 g
		NaHCO3			1.6 g

#2		KBr			0.8 g
		ScCl2			0.34 g

#3		Sodium Silicate		0.4 g

#4		Sodium Fluoride		0.24 g

#5		NH4NO3			0.16 g

#6		Na2HPO4			0.8 g

#7		CaCl2٠2H2O		23.8g

For Stock solution #44 make the following:

Salt				conc. Stock			to make #44 

Na2EDTA				12 g / 200 ml		50 ml / 1000 ml

FeCl2٠6H2O			3.84 g / 20 ml		2 ml / 1000 ml

MgCl2٠6H2O			4.32 g / 20 ml		2 ml / 1000 ml

CoCl2٠6H2O			0.2 g / 20 ml		2 ml / 1000 ml

ZnCl2				0.315 g / 20 ml		2 ml / 1000 ml

CuCl2 				4.8 mg / 34.4 ml	2 ml / 1000 ml

H3BO3				3.42g/100ml		10 ml / 1000 ml

To make ASWJP add into a 1 L liter bottle:

• Add 22.05 g NaCl
• Add 9.8 g MgSO4x7H2O (4.79 g anhydrous)
• Bring up to 900 ml and dissolve.
• Add:

Stock			volume

#1			10 ml
#2			10 ml
#3			1 ml
#4			1 ml
#5			1 ml
#6			1 ml
#7			10 ml
#44			10 ml

For ASWJP PY:

Add 5 g of peptone and 1 g of yeast extract. For plates add 15 g of bacto-agar.

Bring up to 1 liter and autoclave.