ARTIFICIAL SEAWATER (Wyman et al., (1985) Science 230:818-820) Trace metal modifications as in Lindell et al., (1998) J. Bacteriol. 180(7):1878-1886)
To good quality MilliQ water add the following (in grams) for one liter of medium:
NaCl 25 MgCl2.6H20 2 KCl 0.5 NaNO3 0.75 OR NH4Cl 0.11 MgS04.7H20 3.5 CaCl2.2H20 0.5 K2HPO4.3H20 0.03 NaHC03 0.5 HEPES 2.38 OR Tris 1.1
- 1 ml of TMI and TMII (x1000)
- Adjust to pH8.0 and autoclave. (For 4 litres of medium and Tris as buffer – require about 1.5 ml conc HCl, for Hepes as buffer - require about 2.5 ml of 10N NaOH)
Note: If you get a yellow precipitate, you may want to autoclave each of the PO4 and TM mixes separately and add to rest of medium once cooled. This may not be necessary.
- Swirl the medium to bring things back into suspension before aliquoting.
TRACE METALS (as x1000 strength)
TMI g/litre conc (mM) in x1000 stock Ferric Chloride hexahydrate 3.0 11.0 EDTA (disodium salt) 0.5 1.3
TMII g/litre conc (mM) in x1000 stock H3BO3 2.86 46.25 MnCl2.4H20 1.81 9.15 ZnSO4.7H20 0.222 0.77 Na2MoO4.2H20 0.39 1.60 CuSO4.5H20 0.008 0.032 Co(NO3)2.6H20 0.005 0.025
Also see Lindell et al., (1998) - J. Bacteriol. 180(7):1878-1886.
- switched to using HEPES instead of Tris buffer during because Tris interfered with NH4 measurements
- Uses the Va mix (as in Waterbury & Willey (1988): Isolation and growth of marine planktonic cyanobacteria. In: Methods in Enzymology 167:100-105)
media/asw.txt · Last modified: 2007/12/11 12:22 by bwawrik