RNA loading buffer

Prepare DEPC treated water by adding 1 ml DEPC to 1 liter DI and autoclaving twice. Then make loading buffer as follows:

	50 % 	glycerol
	1 mM 	EDTA pH 8.0
	0.4 % 	bromophenol blue
	1 mg/ml	ethidium bromide

Use high grade glycerol to avoid RNAse contamination. Store at -20˚C.