Purification of DNA from CsCl fractions

The following protocol assumes a fraction of 300ul. If other size fractions are collected, adjust volumes accordingly.

• To each 300ul fraction add
  • 600 ul SIP buffer B
  • 1 ul Glycogen
• Vortex well
• Add 600 ul Isopropanol and vortex
• Spin @ 14k rpm for 30 minutes
• Wash pellet with 70% ethanol and dry
• Take up pellet in DI or TE buffer

When I tried this with some Lambda-HindIII marker DNA, I had an average of 95% recovery using this precipitation protocol.

• 50 mM Tris
• 15 mM EDTA pH 8.0