QIAquick PCR Purification Kit Protocol

Add 5 volumes of Buffer PB to 1 volume PCR Reaction (ie. 450 μl to 90 μl PCR amplicon)
Place a QIAquick spin column in a 2 ml collection tube
To bind DNA, apply the sample to the QIAquiick column and centrifuge for 30-60 sec in a microfuge
Discard flow –though liquid. Place the column back into the same tube.
To wash, add 0.75 ml buffer PE to the QIAquick colum and microcentrifuge for 30-60 sec
Discard flow through and place the QIAquick column back in the same tube. Centrifuge the column for an additional 2 min at maximum speed.
Place the QIAquick column into a clean 1.5 ml microcentrifuge tube.
To elute DNA, add 50 μl sterile DI to the center of the QIAquick membrane and centrifuge the column for 1 min.
Proceed to TA Cloning

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