Pipette School

Get a test-tube rack. Put in 3×10 ml conical Sarstedt or equivalent tubes. Please use previously used tubes and non-sterile ones (that’s cheaper). If we have non, then use the sterile ones.

Select a 10 ml pipette, a 5 ml pipette and a 1 ml pipette and an aspirator (suck-ball). Have someone show you how to use the suck-ball.

With a 10 ml pipette, pipette 7 ml in each 10 ml tube. Start at the 10.0 ml mark on the pipette and go down to 3 ml.

Use the 5 ml pipette and pipette 3.5 ml into each of the 10 ml tubes.

Use the 1.0 ml pipette and pipette 1.0 ml into each of the 10 ml tubes. Be sure to use the blow-out function of the suck-ball. Measurement are otherwise inaccurate.

Set up three rows of ten 1.5 ml Eppendorf tubes (non-sterile ones are fine) and three rows of ten 0.5 ml Eppendorf tubes.

Have someone show you how to use a pipetter.

Using the P1000 and blue tips pipette 900 ul into the first row of 1.5 ml tubes.

Using the P200 and yellow tips pipette 150 ul into the second row of 1.5 ml tubes.

Use a P100 to pipette 50 ul into the last row of 1.5 ml tubes.

Using a P20 pipette 1.5 ul into a row of 0.5 ml tubes.

Using a 1-10 ul pipetter and aerosol tips, pipette 1.0 ul into the second row of 0.5 ml tubes.

Pipette 0.5 ul into the last row.

Have someone inspect your tubes.